96-well plate loading for a sequencer protocol

eagle-i ID

Resource Type

Properties

1. Resource Description

   ABI Genetic Analyzers are automated capillary electrophoresis systems that can separate, detect, and analyze up to 16 capillaries of of fluorescent labeled DNA fragments in one run. One run is defined as, at most, 16 sample wells, a 96-well plate can be divided into 6 runs. This protocol outlines how to load plates for efficient sequencing.

2. Uses

   ABI 3100 genetic analyzer

3. Uses

   ABI 3130xl genetic analyzer

4. Uses

   Cycle sequencing

5. Uses

   Nucleic acid fragment analysis

6. Uses

   Sample preparation for assay

7. Used by

   DNA Core Laboratory

8. Website(s)

   https://sites.google.com/a/alaska.edu/iab-corelab/education/protocols/loading-96-well-plates