eagle-i University of Alaska FairbanksUniversity of Alaska Fairbanks
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96-well plate loading for a sequencer protocol

eagle-i ID

http://alaska.eagle-i.net/i/0000012a-2507-bdad-a68e-378080000000

Resource Type

  1. Protocol

Properties

  1. Resource Description
    ABI Genetic Analyzers are automated capillary electrophoresis systems that can separate, detect, and analyze up to 16 capillaries of of fluorescent labeled DNA fragments in one run. One run is defined as, at most, 16 sample wells, a 96-well plate can be divided into 6 runs. This protocol outlines how to load plates for efficient sequencing.
  2. Uses
    ABI 3100 genetic analyzer
  3. Uses
    ABI 3130xl genetic analyzer
  4. Uses
    Cycle sequencing
  5. Uses
    Nucleic acid fragment analysis
  6. Uses
    Sample preparation for assay
  7. Used by
    DNA Core Laboratory
  8. Website(s)
    https://sites.google.com/a/alaska.edu/iab-corelab/education/protocols/loading-96-well-plates
 
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016