Research in the Drew lab focuses on three aspects of hibernation biology. The first involves mechanisms of intrinsic neuroprotective properties of Arctic ground squirrel brain in the hibernating and euthermic state. The second involves mechanisms and cognitive significance of synaptic remodeling observed during hibernation torpor and arousal. The third involves central nervous system regulation of metabolic suppression in hibernation.
"The Brain Slice Keeper has been designed to pre-incubate brain slices prior to transfer into recording chambers."
Microperfusion system for brain slices. Developed by Drew Lab. System is coupled to capillary electrophoresis laser-induced fluorescence assay for amino acid analysis in perfusate; it enables relatively fine temporal resolution of perfusate dynamics.
Attached to Proportional Temperature Controller PTCO3 and Thermo Scientific Barnant Manostat Carter Peristaltic Pump.
Attached to Scientific Systems Design 6-Channel Brain Slice Chamber BSC3-6 and Thermo Scientific Barnant Manostat Carter Peristaltic Pump.
Attached to Proportional Temperature Controller PTCO3 and 6-Channel Brain Slice Chamber BSC3-6
Protocol for preparing acute brain slices for microdialysis and other studies
"MetaFluor® Fluorescence Ratio Imaging Software is the industry standard for monitoring fluorescence ratio imaging applications, bringing microscopists closer to rapid cellular responses inherent to live cells.
MetaFluor Fluorescence Ratio Imaging Software is designed for dual-wavelength intracellular ion measurements. The system provides simultaneous display of the raw data; ratio image; graphs of intensities, ratios, and ion concentrations (e.g., calcium, sodium, potassium, chloride); and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured simultaneously to provide greater insight to ion exchange and intracellular function regardless of dye loading concentrations, conditions, or emission intensities."
"MetaMorph® Microscopy Automation & Image Analysis Software is the industry standard for automated microscope acquisition, device control, and image analysis, bringing microscopists greater understanding of cell morphology, function, and behavior for over 25 years."
"QCapture is a user-friendly image capture program that provides complete control over QImaging digital cameras.
QCapture provides easy control of advanced functions such as pixel binning, ROI (region of interest) selections, gain, offset, white balance, auto-exposure and contrast enhancement."