"The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
* Specially designed Fast block ensures thermal uniformity at top speeds
* Faster ramp rates enable rapid results without compromising extension times or assay quality
* Fast optical plates ensure excellent precision in 10-30 uL reaction volumes
* Use TaqMan Fast Universal PCR Master Mix to run multiple Fast assays on one plate
* Finish more runs per day with the excellent performance equivalent to that offered by the 7500 System"
"GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 96-well GeneAmp® PCR System 9700 is designed for use with 0.2 ml reaction tubes or 96-well reaction plates for all of your routine PCR applications." Two copies of this instrument available.
"The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells on a single platform. Results are delivered within 30-40 minutes in automated, high quality digital data."
Two copies of this instrument available.
Class II, A2
Class II, A2
Protocol for screening serum samples for avian influenza antibodies using ELISA.
Protocol for inoculating, incubating, and harvesting avian influenza virus in allantoic sac fluid of chicken eggs for subsequent RNA extraction and RTPCR.
Adapted from Carol Cardona lab, UC Davis (http://faculty.vetmed.ucdavis.edu/faculty/cjcardona/).
Protocol for sequencing hemagglutinin subtypes using reverse transcription PCR.
Protocol for reverse transcription PCR to differentiate neuraminidase subtypes of avian influenza virus. Qiu et al. 2009. J Virol Meth 155(2):193-198.
Protocol for screening for H5 influenza using real time reverse transcription PCR. Real time RT-PCR for the H5 HA subtype. Spackman, E., Suarez, D.L. 2008. Detection and identification of the H5 hemagglutinin subtype by real-time RT-PCR. In: Spackman, E., editor. Avian Influenza Virus Methods. Totowa, NJ: Humana press. p. 27-34.
Template and protocol for real time and reverse transcription PCR.
Semi-automated extraction of RNA from fecal, cloacal, or oral-pharyngeal swab samples stored in Viral Transport Medium for subsequent RTPCR.
Protocol for sequencing influenza hemagglutinins. Platt et al. 2007 BioTechniques 43:58-62