Wildlife Toxicology Laboratory

Affiliations:

• Center for Alaska Native Health Research
• Department of Biology & Wildlife, University of Alaska
• Department of Chemistry and Biochemistry, University of Alaska
• INBRE Alaska
• Institute of Arctic Biology, University of Alaska

People:

• PI: O'Hara, Todd
  

Member: Lieske, Camilla

Resources:

Instruments

• Beckman Coulter 508 autosampler ( Autosampler )
  

• Beckman System Gold Programmable Detector Module 166 ( High performance liquid chromatography instrument )
  

• Beckman System Gold Programmable Solvent Module 126 ( High performance liquid chromatography instrument )
  

• Brooks Rand methyl mercury analytical system ( Automated mercury analysis system )
  

  System is used for EPA Method 1630.

• Brooks Rand Model III CVAFS HG detector ( Cold vapor atomic fluorescence spectrophotometry detector )
  

  All Brooks Rand devices are part of the same system.

• Brooks Rand total mercury analytical system ( Automated mercury analysis system )
  

  System is used for EPA Method 1631.

• Dionex ASE accelerated solvent extraction system ( Accelerated solvent extraction system )
  

• Eppendorf AG 5810R centrifuge ( Centrifuge )
  

• Iatroscan MK-6/6s Analyzing System ( Gas chromatography detector )
  

• LabConco FreeZone 4.5 Liter Benchtop Freeze Dry System ( Lyophilizer )
  

• Mettler Toledo XP205 Analytical Balance ( Analytical balance )
  

• Mettler Toledo XP504 DeltaRange Analytical Balance ( Analytical balance )
  

• Microtox Model 500 Analyzer ( Photometer )
  

  S/N 1520

• Milestone DMA-80 Direct Mercury Analyzer ( Automated mercury analysis system )
  

  Two copies of this instrument available, one with additional components.

• Olympus BH2 Microscope ( Microscope )
  

• Olympus BX41 Clinical Microscope ( Microscope )
  

• Olympus DP70 Digital Microscope Camera ( Microscope digital camera )
  

• Parr Automatic Isoperibol Calorimeter ( Calorimeter )
  

  Includes 1108 Oxygen Bomb & Bucket.

• PerkinElmer AAnalyst 800 Spectrometer ( Atomic spectrophotometer )
  

  Autosamplers and other components included.

• PerkinElmer AS 800 Autosampler ( Autosampler )
  

• PerkinElmer AS 93 Plus Autosampler ( Autosampler )
  

• PerkinElmer FIAS 100 Flow Injection System ( Flow injection system )
  

• PerkinElmer Multiwave 3000 (Anton Paar) ( Microwave synthesis system )
  

• Polytron PT 10-35 Tissue Homogenizer ( Homogenizer )
  

• Precision Model 45EG Gravity Convection Oven ( Gravity convection oven )
  

• SpectraMax 340PC [384] ( Spectrophotometer )
  

• Tekran CVAFS Mercury Detector 2500 ( Cold vapor atomic fluorescence spectrophotometry detector )
  

• Thermolyne Type 37900 Culture Incubator ( Incubator )
  

• VWR CryoPro cell storage device ( Cell storage device )
  

Protocols

• Calorimetry protocol ( Protocol )
  

  Specifies procedure for determining caloric content of a sample using bomb calorimetry.

• Freeze drying protocol ( Protocol )
  

  Specifies procedures for freeze drying samples before further analysis or storage.

• Haptoglobin protocol ( Protocol )
  

  Specifies procedures for analysis of haptoglobin levels in blood plasma samples.

• Hematology protocol ( Protocol )
  

  Specifies procedures for hematology screening of whole blood samples

• Histology protocol ( Protocol )
  

  Specifies procedures for fixing, embedding, sectioning, staining of tissue samples for subsequent microscopy

• Methyl mercury analysis protocol ( Protocol )
  

  Specifies procedures for determining level of methyl mercury in tissue sample.

• Serology protocol ( Protocol )
  

  Specifies procedures for serology profiles.

• Stable isotope preparation protocol ( Protocol )
  

  Specifies procedures for preparing samples for stable isotope analysis.

• Tissue digestion for amino acid analysis protocol ( Protocol )
  

  Specifies procedures to digest tissue samples for analysis of amino acid composition

• Tissue digestion for mercury analysis protocol ( Protocol )
  

  Specifies procedures to digest tissue samples for subsequent analysis of mercury levels.

• Tissue preparation for fatty acid analysis protocol ( Protocol )
  

  Specifies procedures for preparing samples for subsequent analysis for fatty acid composition.

• Tissue preparation for organochlorine analysis protocol ( Protocol )
  

  Specifies procedures for preparing samples for subsequent analysis of organochlorine contamination.

• Total mercury analysis protocol ( Protocol )
  

Software

• DP Controller ( Software )
  

  Image processing software

• Image Pro ( Software )
  

  "Powerful 2D and 3D image processing, enhancement, and analysis software with extensive measurement and customization features"

• SigmaPlot ( Software )
  

  "SigmaPlot graphing software from SYSTAT takes you beyond simple spreadsheets to help you show off your work clearly and precisely. With SigmaPlot, you can produce high-quality graphs without spending hours in front of a computer. SigmaPlot offers seamless Microsoft Office integration, so you can easily access data from Microsoft Excel spreadsheets and present your results in Microsoft PowerPoint® presentations."

• SYSTAT ( Software )
  

  "SYSTAT is a versatile and comprehensive statistical software package. It employs a staggering range of powerful techniques to help conduct many types of research.
  
  Novice statistical users can use SYSTAT’s menu-driven interface to conduct simple analyses and produce beautiful 2D and 3D graphics for presentations or reports.
  
  Those in need of advanced analysis can use SYSTAT’s more sophisticated statistical routines, and create plots and charts that are instantly ready for publication. SYSTAT’s menu and command interfaces are interlinked, allowing users the option of whichever method works best for them."