Fragment Analysis (microsats, AFLP, T-RFLP)

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   The user provides premixed samples (PCR product , size standard and HiDi Formamide) in a 96-well plate. The Core Lab tech then loads and runs the samples. All PCR supplies and size standards are provided by the user. HiDi Formamide is provided by the Core Lab at no extra cost. The Core lab only accepts pre-mixed fragment samples for fragment analysis. It does not affect the price if markers are multiplexed in each well; only the number of wells matters. The User enters sample names into the computer, otherwise the samples are labeled only by well number. Ask in the Core Lab for a sample sheet template if you want to enter names on your computer.

3. Service Fee URL

   https://sites.google.com/a/alaska.edu/iab-corelab/services-1/services

4. Related Resource

   ABI 3100 genetic analyzer

5. Related Resource

   ABI 3130xl genetic analyzer

6. Access Restriction(s)

   Available only to researchers in Alaska.

7. Service Provided by

   DNA Core Laboratory

8. Related Protocol(s)

   Protocol for submitting samples for fragment analysis

9. Related Technique

   Restriction fragment length polymorphism electrophoresis

10. Related Technique

    Microsatellite analysis

11. Related Technique

    Nucleic acid fragment analysis

12. Related Technique

    Amplified Fragment Length Polymorphism PCR

13. Related Technique

    Terminal Restriction Fragment Length Polymorphism

14. ERO_0001053

    Yes