NanoDrop ND-1000 Spectrophotometer

eagle-i ID

Resource Type

Properties

1. Resource Description

   Used for DNA and RNA quantification. The NanoDrop ND-1000 is a full-spectrum (220-750nm) spectrophotometer that can measure concentrations in 1-2ul of sample with good accuracy and reducibility. It has the capability to measure highly concentrated samples without dilution (75x higher concentrations than can be measured in a standard cuvette spec). To operate the NanoDrop you pipette 1 or 2ul of sample onto the end of a fiber optic cable (receiving). A second fiber optic cable (source) is lowered down on an arm and causes the liquid to form a column between the two cables. A pulsed xenon flash lamp provides the light source and a spectrometer using a linear CCD array analyzes the light after it passes through the sample. A laptop is attached with software that controls the NanoDrop and collects and stores the data at which point you can save the files or print them out. The NanoDrop ND-1000 can be used to measure: Nucleic acid concentration and quality of nucleic acid samples up to 3700ng/ul (dsDNA) without dilution. Fluorescent dye labeling density of nucleic acid microarray samples Purified protein analysis (A280) up to 100 mg.ml (BSA) Bradford Assay analysis of protein Cell density measurements General UV-Vis spectrophotometry

2. Manufacturer

   Thermo Fisher Scientific

3. Inventory Number

   204987

4. Model Number

   ND-1000

5. Website(s)

   http://www.nanodrop.com/Library/nd-1000-v3.7-users-manual-8.5x11.pdf

6. Website(s)

   https://sites.google.com/a/alaska.edu/iab-corelab/equipment/spectrophotometers/nanodrop-nd-1000-spectrophotometer

7. Location

   DNA Core Laboratory