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NanoDrop ND-1000 Spectrophotometer

eagle-i ID


Resource Type

  1. Spectrophotometer


  1. Resource Description
    Used for DNA and RNA quantification. The NanoDrop ND-1000 is a full-spectrum (220-750nm) spectrophotometer that can measure concentrations in 1-2ul of sample with good accuracy and reducibility. It has the capability to measure highly concentrated samples without dilution (75x higher concentrations than can be measured in a standard cuvette spec). To operate the NanoDrop you pipette 1 or 2ul of sample onto the end of a fiber optic cable (receiving). A second fiber optic cable (source) is lowered down on an arm and causes the liquid to form a column between the two cables. A pulsed xenon flash lamp provides the light source and a spectrometer using a linear CCD array analyzes the light after it passes through the sample. A laptop is attached with software that controls the NanoDrop and collects and stores the data at which point you can save the files or print them out. The NanoDrop ND-1000 can be used to measure: Nucleic acid concentration and quality of nucleic acid samples up to 3700ng/ul (dsDNA) without dilution. Fluorescent dye labeling density of nucleic acid microarray samples Purified protein analysis (A280) up to 100 mg.ml (BSA) Bradford Assay analysis of protein Cell density measurements General UV-Vis spectrophotometry
  2. Manufacturer
    Thermo Fisher Scientific
  3. Inventory Number
  4. Model Number
  5. Website(s)
  6. Website(s)
  7. Location
    DNA Core Laboratory
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016